nick.lockyer @ manchester.ac.uk
tel: +44 (0)161 306 4479
- PhD on Laser Ionisation Mass Spectrometry of Biomolecules, UMIST, 1996
- BSc in Chemistry, UMIST
- Special Research Fellowship for the ‘Development of a Chemical Microscope‘ from the Leverhulme Trust.
My research interests focus on the development and application of surface analysis and chemical imaging particularly ToF-SIMS and laser-based mass spectrometry. There are three themes to this research: fundamentals, instrument development and applications research. The Life Sciences provide an exciting field in which to develop new applications, which in turn drive developments in methodology and technology. We are currently seeking to exploit these advances through a variety of collaborations with fellow academics, industrial partners and healthcare professionals.
Research topics: Analytical Chemistry; Instrumentation; Chemical imaging; Ion-solid interactions; Laser-matter interactions.
Figure 1. The impact of keV ions or laser photons on a solid surface results in the ejection of ions and neutrals that reflect the detailed surface chemistry of the impact region. Scanning the focused probe beam over the sample leads to chemical images. The emitted ions can be analysed and detected directly by mass spectrometry e.g. ToF-SIMS. A complementary approach is to ‘post-ionise’ the neutrals, for example with an intense laser pulse. Figure 2.
Classification of cancer cell lines using ToF-SIMS and multivariate analysis. Red is training data, blue is blind-test data. Confidence limits of 95% (outer ellipse) and 99% (inner ellipse) are shown. 1 = PC-3 ( human prostatic adenocarcinoma metastatic to bone); 2 = LNCaP (human prostatic adenocarcinoma metastatic to lymph node); 3 = PNT2 (adult prostatic epithelial cells). Reproduced from M.J. Baker, E. Gazi, M.D. Brown, N.W. Clarke, J.C. Vickerman
and N.P. Lockyer, Applied Surface Science 255 (2008) 1084-1087
Current Research Projects
- Development of ToF-SIMS instrumentation for biochemical microscopy
Collaborators: J.C. Vickerman (UoM School of Chemical Engineering & Analytical Science & MIB)
- Laser post-ionisation
Collaborators: N. Winograd (Penn State, Dept of Chemistry)
- Development of ToF-SIMS for metabolite profiling
Collaborators: R. Goodacre (UoM School of Chemistry & MIB)
- Imaging single biological cells using ToF-SIMS
Collaborators: J. Lovric, N. Papalopulu (UoM Faculty of Life Science)
- The application of ToF-SIMS in cancer biology, diagnosis and therapy
Collaborators: K. Williams (UoM School of Pharmacy & Pharmaceutical Sciences); A. McMahon (UoM School of Cancer Studies and Wolfson Molecular Imaging Centre); P. Gardner (UoM, School of Chemical Engineering & Analytical Science), N. Clarke & M. Brown (UoM School of Cancer and Enabling Sciences & CRUK Paterson Institute for Cancer Research), G. Cruickshank, S. Green, A. Detta (University Hospitals Birmingham)
- ToF-SIMS imaging of Alzheimer Disease brain tissues
Collaborators: H. Boutin (UoM Faculty of Life Science);
EPSRC, BBSRC, CRUK