Senior Experimental Officer
Honorary Lecturer in Cheminformatics
Honorary Lecturer in Analytical Science
alex.henderson @ manchester.ac.uk
My research interests lie in data reduction, working mainly with the results from ToF-SIMS (time of flight secondary ion mass spectrometry) and IR (infra red spectroscopy) experiments in cell chemistry.
Chemometric and cheminformatic approaches are applied to spectra, 2D chemical images and 3D chemical hypercubes to understand the complex chemistries involved in cell structure, metabolism and drug interaction. Work carried out in parallel aims to develop visualisation tools for chemists and biologists working in this area.
Having been in the group for quite a while I find myself involved in most of the projects we have running at any one time. In addition to programming, I find myself helping out around the group, assisting with experimental technique and equipment repair.
In addition to my work in the SIMS field I also work on FTIR data of tissue as a member of Peter Gardner’s research group.
I recently taught Instrumentation for Separation Science which was a module of our Analytical and Separation Science Masters programme. My lectures covered the interpretation of mass spectrometric data.
Previously I taught Database Design and Programming on the School of Chemistry’s Cheminformatics Masters programme.
I am a director of SurfaceSpectra Ltd. and also write their software.
Recognised UK National Expert
- TC 201/SC 1 Terminology
- TC 201/SC 2 General procedures
- TC 201/SC 3 Data management and treatment
- TC 201/SC 6 Secondary ion mass spectrometry
Conference organising committees
- 59th IUVSTA Workshop Surface Chemical Analysis – Improving Data Interpretation by Multivariate and Informatics Techniques
Island of Trinidad, 2010 (Scientific Committee)
- The 6th International SPEC conference
Manchester 2010 (Local Organising Committee)
- The 15th International Conference on Secondary Ion Mass Spectrometry (SIMS XV)
Manchester 2005 (Local Organising Committee)
Here are links to articles I have found particularly interesting/useful.
- How Many Structures Can You Generate From A Molecular Formula?
Antony Williams, a member of the ChemSpider initiative.
Here are links to articles in which I have been acknowledged.
|Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectral discrimination of brain tumour severity from serum samples
James R. Hands, Konrad M. Dorling, Peter Abel, Katherine M. Ashton, Andrew Brodbelt, Charles Davis, Timothy Dawson, Michael D. Jenkinson, Robert W. Lea, Carol Walker and Matthew J. Baker
Journal of Biophotonics 7(3-4) (2014) 189–199
|Effect of Substrate Choice and Tissue Type on Tissue Preparation for Spectral Histopathology by Raman Microspectroscopy
Leanne M Fullwood, David M Griffiths, Katherine Ashton, Timothy Dawson, Robert W Lea, Charles Davis, Franck Bonnier, Hugh Byrne and Matthew J Baker
Analyst 139 (2014) 446-454
|Single Cell Lipidomics: Characterizing and Imaging Lipids on the Surface of Individual Aplysia Californica Neurons with Cluster SIMS
Melissa K. Passarelli, Andrew G. Ewing and Nicholas Winograd
Analytical Chemistry 85(4) (2013) 2231–2238
|Imaging of metabolites using secondary ion mass spectrometry
Emily G. Armitage, Helen L. Kotze, Nicholas P. Lockyer
Metabolomics 9 (2013) S102-S109
|ToF-SIMS PC-DFA analysis of prostate cancer cell lines.
M.J. Baker, E. Gazi, M.D. Brown, N.W. Clarke, J.C. Vickerman and N.P. Lockyer
Appl. Surf. Sci. 255 (2008) 1084-1087
|TOF-SIMS investigation of Streptomyces coelicolor, a mycelial bacterium.
Seetharaman Vaidyanathan, John S. Fletcher, Nicholas P. Lockyer and John C. Vickerman
Appl. Surf. Sci. 255 (2008) 922-925
|Subsurface Biomolecular Imaging of Streptomyces coelicolor Using Secondary Ion Mass Spectrometry.
Seetharaman Vaidyanathan, John S. Fletcher, Roy Goodacre, Nicholas P. Lockyer, Jason Micklefield, and John C. Vickerman
Analytical Chemistry 80 (2008) 1942-1951
|Depth Profiling Brain Tissue Sections with a 40 keV C60+ Primary Ion Beam.
Emrys A. Jones, Nicholas P. Lockyer, John C. Vickerman
Analytical Chemistry 80 (2008) 2125-2132
|TOF-SIMS 3D Biomolecular Imaging of Xenopus laevis Oocytes Using Buckminsterfullerene (C60) Primary Ions.
John S. Fletcher, Nicholas P. Lockyer, Seetharaman Vaidyanathan, John C. Vickerman
Analytical Chemistry 79 (2007) 2199-2206
Here are links to grants on which I have been funded.
|2019||New Developments in Quantitative 3D Chemical Imaging
Nick Lockyer (PI)
April 2019 – March 2022
|2016||Development of Multiplexed ToF-SIMS Instrumentation
Nick Lockyer (PI)
August 2016 – July 2019
|2014||Clinical Infrared and Raman Spectroscopy Network (CLIRSPEC)
Peter Gardner (PI), Matthew Baker (UCLAN)
February 2014 – January 2017
|2013||Towards disease diagnosis through spectrochemical imaging of tissue architecture.
Peter Gardner (PI)
August 2013 – July 2016
|2013||Automated breast cancer screening: An interdisciplinary pilot study bringing together chemical imaging, cancer pathology and machine learning to produce a next generation healthcare technology.
Peter Gardner (PI), Jonathan Shapiro, Kaye Williams and Michael Lisanti
University of Manchester Research Institute (UMRI) – Pump Priming Programme £26k
|2013||Application of single cell metabolite profiling to optimisation of stem cell bioprocessing
A.J. Dickson (PI), K.E. Cosgrove, M.J. Dunne, R. Goodacre, N.P. Lockyer
August 2013 – July 2016
|2011||MaDAM into Sustainable Service (MiSS)
Lorraine Beard, PI (Head of Information Services, JRUL), Project Manager June Finch
JISC £245k + £730k institutional support
October 2011 – March 2013 (extension of the MaDAM project)
|2011||Infrared Imaging for Diagnosis and Prediction of the Biopotental of Low and Intermediate Risk Prostate Cancer
Gardner (PI), Brown, Clarke
April 2011 – March 2014
|2011||MIB CDFA – Application of novel imaging technologies towards quantitative analytical phenotyping of single cells
Flitsch (PI), Goddard, Vickerman, Bayat, Westerhoff, Yuan, Webb, Gardner, Fielden, Goodacre, Lockyer, Fletcher, Blanch
January 2011 – January 2012
|2009||A new dynamic in mass spectral imaging for biological systems
Vickerman (PI), Lockyer, Fletcher
June 2009 – May 2012
|2009||A combined micro fluidic single cell SRIR microscopy stage for use at Diamond
Gardner (PI), Cinque, Fielden, Goddard
January 2009 – December 2012
|2007||Enhancing new developments in ToF-SIMS through researcher exchanges
Vickerman, Lockyer, Webb (Surrey)
EPSRC Collaborating for Success Through People Award £110k
|2005||New Developments in ToF-SIMS Surface Mass Spectrometry with ATR-IR Spectroscopy
Vickerman (PI), Lockyer, Gardner, Horn, Goodacre; Webb and Kirkby (Surrey).
EPSRC Critical Mass Award £2M
October 2005 – Sept 2009
|2004||A High Resolution ToF-SIMS Microscope for Biology
Vickerman (PI), Lockyer, Goodacre and Kell.
August 2004 – December 2007
|2000||Development of ToF-SIMS instrumentation and methodology for chemical microscopy of cellular systems
Vickerman, Lockyer and Stephens
EPSRC Life Sciences Interface £312k
June 2000 – June 2003
|1996||Wiley Static SIMS Library – Ongoing funding for updates £13,000/year|
|1994||Extension to Static SIMS Library Grant July 1994 to May 1995 – John Wiley & Sons, £17,000|
|1992||Support from John Wiley & Sons for the launch costs (>£50k) of an International Static SIMS Database.|
Mass Spectrometry @ Manchester
Peter Gardner’s Research Group
Paul Bassan’s company: London Spectroscopy