Dr Nick Lockyer

Dr Nick Lockyer

READER (ASSOCIATE PROF)

nick.lockyer @ manchester.ac.uk

tel: +44 (0)161 306 4479


  • PhD on Laser Ionisation Mass Spectrometry of Biomolecules, UMIST, 1996
  • BSc in Chemistry, UMIST
  • Special Research Fellowship for the ‘Development of a Chemical Microscope‘ from the Leverhulme Trust.

My research interests focus on the development and application of surface analysis and chemical imaging particularly ToF-SIMS and laser-based mass spectrometry. There are three themes to this research: fundamentals, instrument development and applications research. The Life Sciences provide an exciting field in which to develop new applications, which in turn drive developments in methodology and technology. We are currently seeking to exploit these advances through a variety of collaborations with fellow academics, industrial partners and healthcare professionals.

Research topics: Analytical Chemistry; Instrumentation; Chemical imaging; Ion-solid interactions; Laser-matter interactions.

Laser postionisation schematic

Figure 1. The impact of keV ions or laser photons on a solid surface results in the ejection of ions and neutrals that reflect the detailed surface chemistry of the impact region. Scanning the focused probe beam over the sample leads to chemical images.  The emitted ions can be analysed and detected directly by mass spectrometry e.g. ToF-SIMS.  A complementary approach is to ‘post-ionise’ the neutrals, for example with an intense laser pulse.
Classification of cancer cell lines Figure 2. Classification of cancer cell lines using ToF-SIMS and multivariate analysis. Red is training data, blue is blind-test data. Confidence limits of 95% (outer ellipse) and 99% (inner ellipse) are shown. 1 = PC-3 ( human prostatic adenocarcinoma metastatic to bone); 2 =  LNCaP (human prostatic adenocarcinoma metastatic to lymph node);  3 = PNT2 (adult prostatic epithelial cells). Reproduced from M.J. Baker, E. Gazi, M.D. Brown, N.W. Clarke, J.C. Vickerman  and N.P. Lockyer, Applied Surface Science 255 (2008) 1084-1087.

Chemical imaging of a frog embryo

Current Research Projects

  • Development of ToF-SIMS instrumentation for 2D and 3D chemical imaging
    Collaborators: Ionoptika Ltd (Southampton)
  • Laser post-ionisation of sputtered neutrals
    Collaborators: N. Winograd (Penn State, Dept of Chemistry)
  • Ambient mass spectrometry in biotechnology and biomedicine 
    Collaborators: P. Barran, E. Takano (UoM, School of Chemistry), Waters Corp.
  • Development of mass spectrometry imaging for metabolite profiling in single cells
    Collaborators: R. Goodacre (University of Liverpool), A. Dickson (UoM School of Chemical Engineering & Analytical Science)
  • The application of ToF-SIMS in cancer biology, diagnosis and therapy
    Collaborators: K. Williams (UoM School of Pharmacy & Pharmaceutical Sciences); A. McMahon (UoM School of Cancer Studies and Wolfson Molecular Imaging Centre); P. Gardner  (UoM, School of Chemical Engineering & Analytical Science), N. Clarke & M. Brown (UoM School of Cancer and Enabling Sciences & CRUK Paterson Institute for Cancer Research), G. Cruickshank, S. Green, A. Detta (University Hospitals Birmingham)

Recent Funding Sources

EPSRC, BBSRC, CRUK

Recent Publications [All Publications]

108 The influence of polyatomic primary ion chemistry on matrix effects in secondary ion mass spectrometry analysis
Afnan M. Alnajeebi, John C. Vickerman and Nicholas P. Lockyer
Rapid Communications in Mass Spectrometry 32 (2018) 1962-1970
107 Probing the action of a novel anti-leukaemic drug therapy at the single cell level using modern vibrational spectroscopy techniques
Joanna L. Denbigh, David Perez-Guaita, Robbin R. Vernooij, Mark J. Tobin, Keith R. Bambery, Yun Xu, Andrew D. Southam, Farhat L. Khanim, Mark T. Drayson, Nicholas P. Lockyer, Royston Goodacre & Bayden R. Wood
Scientific Reports 7 (2017) 2649
106 Quartz Crystal Microbalance Assay of Clinical Calcinosis Samples and Their Synthetic Models Differentiates the Efficacy of Chelation-Based Treatments
Fan Fei, Andrzej Gallas, Yun-Chuan Chang, Yikun Rao, Alan Christy Hunter, Richard E. P. Winpenny, Ariane L. Herrick, Nicholas P. Lockyer and Christopher F. Blanford
ACS Appl. Mater. Interfaces 9 (2017) 27544–27552
105 Fossilization of melanosomes via sulfurization
Maria E. McNamara, Bart E. van Dongen, Nick P. Lockyer, Ian D. Bull and Patrick J. Orr
Palaeontology 59 (2016) 337-350
104 Matrix effects in biological SIMS using cluster ion beams of different chemical composition
Afnan M. Alnajeebi, John C. Vickerman and Nicholas P. Lockyer
Biointerphases 11 (2016) 02A317
103 Peptide Fragmentation and Surface Structural Analysis by Means of ToF-SIMS Using Large Cluster Ion Sources
Yuta Yokoyama, Satoka Aoyagi, Makiko Fujii, Jiro Matsuo, John S. Fletcher, Nicholas P. Lockyer, John C. Vickerman, Melissa K. Passarelli, Rasmus Havelund and Martin P. Seah
Analytical Chemistry 88 (2016) 3592–3597
102 Evaluation of biomolecular distributions in rat brain tissues by means of ToF-SIMS using a continuous beam of Ar clusters
Shusuke Nakano, Yuta Yokoyama, Satoka Aoyagi, Naoyuki Himi, John S. Fletcher, Nicholas P. Lockyer, Alex Henderson and John C. Vickerman
Biointerphases 11 (2016) 02A307
101 SIMS Matrix Effects of Biological Molecules under Cluster Ion Beam Bombardment
Afnan M. Alnajeebi, Sadia Sheraz née Rabbani, John C. Vickerman and Nicholas P. Lockyer
Proceedings of the Eighth Saudi Students Conference in the UK (2015) 437-444
100 Mass spectrometric imaging of brain tissue by time-of-flight secondary ion mass spectrometry – How do polyatomic primary beams C60+, Ar2000+, water-doped Ar2000+ and (H2O)6000+ compare?
Irma Berrueta Razo, Sadia Sheraz (née Rabbani), Alex Henderson, Nicholas P. Lockyer and John C. Vickerman
Rapid Commun. Mass Spectrom. 29 (2015) 1851–1862
99 Enhancing Ion Yields in TOF-SIMS - A Comparative Study of Argon and Water Cluster Primary Beams
Sadia Sheraz, Irma Berrueta Razo, Taylor P. Kohn, Nicholas P. Lockyer, and John C. Vickerman
Analytical Chemistry 87 (2015) 2367–2374

Books

  • N.P. Lockyer,  Laser Post-ionisation for Elemental and Molecular Surface Analysis in ToF-SIMS – Surface Analysis by Mass Spectrometry, Second Edition, Eds. J.C. Vickerman and D. Briggs, SurfaceSpectra (Manchester) and IM Publications (Chichester)
  • N.P. Lockyer “Static Secondary Ion Mass Spectrometry for Biological and Biomedical ResearchMethods Mol. Biol. 369 (2007) 543-567

Selected Presentations

  • INVITED,  New prospects for metabolic profiling and imaging using secondary ion mass spectrometry, Metabomeeting 2012, Manchester, September 2012.
  • New prospects for post-ionisation mass spectrometry using ultrafast laser pulses, 19th International Mass Spectrometry Conference, Kyoto, Japan, September 2012
  • INVITED N.P. Lockyer, British Mass Spectrometry Society 2011, Cardiff, UK, Sept. 2011.
  • KEYNOTE “Developments in biological analysis and imaging using Secondary Ion Mass Spectrometry” Metabolomics 2011, Cairns, Australia, June 2011.
  • INVITED “3D chemical imaging with cluster SIMS”, 20th Ion Beam Analysis conference, Itapema, Brazil , April 2011.

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