My current research focuses on exploring and testing new primary ion sources developed in collaboration with Ionoptika to expand the capabilities of ToF-SIMS for the analysis of organic and biological samples. Although current primary ion sources available, particularly C60 allow molecular depth profiling and 3D molecular imaging to be performed, not all samples can be analysed using these beams. Thus it is important to explore the potential of other candidates. I am currently working with an argon giant cluster ion beam to assess its ability for molecular depth profiling in terms of damage accumulation, sputter rates and secondary ion yields in comparison with other ion sources. Furthermore the potential of this ion source for molecular imaging is also being explored.
I am also working on another research project involving mass spectrometric imaging for 2D and 3D cellular characterisation of single cells and tissue sections using the newly developed 3D Chemical Imager SIMS instrument.
My PhD was based on advancing the technique ToF-SIMS for the analysis of single cells on a sub-cellular scale. This involved proving the concept and potential of a novel SIMS instrument, the J105 3D Chemical Imager, developed in collaboration with Ionoptika. Initially standard organic samples were used for analysis to show the current performance of the J105 with respect to a conventional ToF-SIMS instrument. This was followed by analysis of single cells in 2D and 3D modes. The data generated show molecular localization on a sub-cellular scale with high mass resolution.
Enhanced Ion Yields Using High Energy Water Cluster Beams for Secondary Ion Mass Spectrometry Analysis and Imaging
Sadia Sheraz (née Rabbani), Hua Tian, John C. Vickerman, Paul Blenkinsopp, Nicholas Winograd and Peter Cumpson
Analytical Chemistry 91 (2019) 9058–9068
SIMS Matrix Effects of Biological Molecules under Cluster Ion Beam Bombardment
Afnan M. Alnajeebi, Sadia Sheraz née Rabbani, John C. Vickerman and Nicholas P. Lockyer
Proceedings of the Eighth Saudi Students Conference in the UK (2015) 437-444
Mass spectrometric imaging of brain tissue by time-of-flight secondary ion mass spectrometry – How do polyatomic primary beams C60+, Ar2000+, water-doped Ar2000+ and (H2O)6000+ compare?
Irma Berrueta Razo, Sadia Sheraz (née Rabbani), Alex Henderson, Nicholas P. Lockyer and John C. Vickerman
Rapid Commun. Mass Spectrom. 29 (2015) 1851–1862
Enhancing Ion Yields in TOF-SIMS - A Comparative Study of Argon and Water Cluster Primary Beams
Sadia Sheraz, Irma Berrueta Razo, Taylor P. Kohn, Nicholas P. Lockyer, and John C. Vickerman
Analytical Chemistry 87 (2015) 2367–2374
Prospect of increasing secondary ion yields in ToF-SIMS using water cluster primary ion beams
S. Sheraz (née Rabbani), A. Barber, I. Berrueta Razo, J. S. Fletcher, N.P. Lockyer and J. C. Vickerman
Surf. Interface Anal. 46 (2014) 51–53
Comparing C60+ and (H2O)n+ clusters for mouse brain tissue analysis
Irma Berrueta Razo, Sadia Sheraz, Alex Henderson, Nicholas P. Lockyer and John C. Vickerman
Surf. Interface Anal. 46 (2014) 136–139
Peptide structural analysis using continuous Ar cluster and C60 ion beams
Satoka Aoyagi, John S. Fletcher, Sadia Sheraz (Rabbani), Tomoko Kawashima, Irma Berrueta Razo, Alex Henderson, Nicholas P. Lockyer, John C. Vickerman
Analytical and Bioanalytical Chemistry 405 (2013) 6621-6628
Enhancing Secondary Ion Yields in Time of Flight-Secondary Ion Mass Spectrometry Using Water Cluster Primary Beams
Sadia Sheraz née Rabbani, Andrew Barber, John S. Fletcher, Nicholas P. Lockyer, and John C. Vickerman
Analytical Chemistry 85 (2013) 5654–5658
Comparison of C60 and GCIB primary ion beams for the analysis of cancer cells and tumour sections
John S. Fletcher, Sadia Rabbani, Andrew M. Barber, Nicholas P. Lockyer, John C. Vickerman
Surf. Interface Anal. 45 (2013) 273-276
TOF-SIMS with Argon Gas Cluster Ion Beams: A Comparison with C60+.
Sadia Rabbani, Andrew M. Barber, John S. Fletcher, Nicholas P. Lockyer, John C. Vickerman
Analytical Chemistry 83 (2011) 3793–3800
Exploring subcellular imaging on the buncher-ToF J105 3D chemical imager.
S. Rabbani, J. S. Fletcher, N. P. Lockyer and J. C. Vickerman
Surf. Interface Anal. 43 (2011) 380-384
Three-dimensional mass spectral imaging of HeLa-M cells - sample preparation, data interpretation and visualisation.
John S. Fletcher, Sadia Rabbani, Alex Henderson, Nicholas P. Lockyer, John C. Vickerman
Rapid Commun. Mass Spectrom. 25 (2011) 925-932
A New Dynamic in Mass Spectral Imaging of Single Biological Cells.
John S. Fletcher, Sadia Rabbani, Alex Henderson, Paul Blenkinsopp, Steve P. Thompson, Nicholas P. Lockyer, and John C. Vickerman
Analytical Chemistry 80 (2008) 9058-9064
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