Dr Katherine Hollywood

Katherine Hollywood

Research Associate

katherine.hollywood @ manchester.ac.uk

Katherine Hollywood ORCID Account

I am currently employed on a BBSRC Bioprocessing Research Industry Club (BRIC) funded project implementing the application of single cell metabolite profiling to the optimisation of stem cell bioprocessing. The project aims to use single cell imaging techniques including SIMS, MALDI-MS & Raman spectroscopy to define the metabolic profile that is characteristic of stem cells progressing towards distinct cellular fates and profiles that are indicative of cells at different stages of differentiation.

In my previous postdoctoral position I was the lead scientist for all aspects of targeted and untargeted LC-MS-based metabolomics including sample analysis, instrument maintenance and analytical troubleshooting. I conducted a diverse range of profiling metabolomic experiments undertaken on a LTQ Orbitrap Velos (Thermo Scientific) analysing an array of biological sample matrices including, serum, plasma, urine, various tissues and CSF. I also developed a number of targeted LC-MS methods for a range of complex analytes utilising a TSQ Vantage QQQ (Thermo Scientific). Additionally, I was responsible for the data reduction and analysis of complex data sets and subsequent summarising of results.

I greatly enjoy working in a multidisciplinary environment and bridging the gap between instrumentation and clinical research.


17 Elevation of brain glucose and polyol-pathway intermediates with accompanying brain-copper deficiency in patients with Alzheimer’s disease: metabolic basis for dementia
Jingshu Xu, Paul Begley, Stephanie J. Church, Stefano Patassini, Selina McHarg, Nina Kureishy, Katherine A. Hollywood, Henry J. Waldvogel, Hong Liu, Shaoping Zhang, Wanchang Lin, Karl Herholz, Clinton Turner, Beth J. Synek, Maurice A. Curtis, Jack Rivers-Auty, Catherine B. Lawrence, Katherine A. B. Kellett, Nigel M. Hooper, Emma R. L. C. Vardy, Donghai Wu, Richard D. Unwin, Richard L. M. Faull, Andrew W. Dowsey and Garth J. S. Cooper
Scientific Reports 6 (2016) 27524
16 Characterisation of the metabolome of ocular tissues and post-mortem changes in the rat retina
Shi Z. Tan, Graham Mullard, Katherine A. Hollywood, Warwick B. Dunn and Paul N. Bishop
Experimental Eye Research 149 (2016) 8–15
15 Graded perturbations of metabolism in multiple regions of human brain in Alzheimer's disease: Snapshot of a pervasive metabolic disorder
Jingshu Xu, Paul Begley, Stephanie J. Church, Stefano Patassini, Katherine A. Hollywood, Mia Jüllig, Maurice A. Curtis, Henry J. Waldvogel, Richard L.M. Faull, Richard D. Unwin and Garth J.S. Cooper
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1862 (2016) 1084–1092
14 Introduction to metabolomics and its applications in ophthalmology
S Z Tan, P Begley, G Mullard, K A Hollywood and P N Bishop
Eye 30 (2016) 773–783
13 Meat, the metabolites: an integrated metabolite profiling and lipidomics approach for the detection of the adulteration of beef with pork
Drupad K. Trivedi, Katherine A. Hollywood, Nicholas J. W. Rattray, Holli Ward, Dakshat K. Trivedi, Joseph Greenwood, David I. Ellis and Royston Goodacre
Analyst 141 (2016) 2155-2164
12 Metabolic Dysfunction Is Restricted to the Sciatic Nerve in Experimental Diabetic Neuropathy
Oliver J. Freeman, Richard D. Unwin, Andrew W. Dowsey, Paul Begley, Sumia Ali, Katherine A. Hollywood, Nitin Rustogi, Rasmus S. Petersen, Warwick B. Dunn, Garth J.S. Cooper and Natalie J. Gardiner
Diabetes 65 (2015) 228-238
11 Exploring the mode of action of dithranol therapy for psoriasis: a metabolomic analysis using HaCaT cells
Katherine A. Hollywood, Catherine L. Winder, Warwick B. Dunn, Yun Xu, David Broadhurst, Christopher E. M. Griffiths and Royston Goodacre
Molecular BioSystems 11 (2015) 2198-2209
10 A HPLC-SRM-MS based method for the detection and quantification of methotrexate in urine at doses used in clinical practice for patients with rheumatological disease: a potential measure of adherence
J. Bluett, I. Riba-Garcia, K. Hollywood, S. M. M. Verstappen, A. Barton and R. D. Unwin
Analyst 140 (2015) 1981-1987
9 Making colourful sense of Raman images of single cells
Lorna Ashton, Katherine A. Hollywood and Royston Goodacre
Analyst 140 (2015) 1852-1858
8 The influence of scaling metabolomics data on model classification accuracy
Piotr S. Gromski, Yun Xu, Katherine A. Hollywood, Michael L. Turner and Royston Goodacre
Metabolomics 11 (2015) 684-695
7 A new strategy for MS/MS data acquisition applying multiple data dependent experiments on Orbitrap mass spectrometers in non-targeted metabolomic applications
Graham Mullard, James W. Allwood, Ralf Weber, Marie Brown, Paul Begley, Katherine A. Hollywood, Martin Jones, Richard D. Unwin, Paul N. Bishop, Garth J. S. Cooper, Warwick B. Dunn
Metabolomics 11 (2015) 1068-1080
6 Metabolomic analysis of rat serum in streptozotocin-induced diabetes and after treatment with oral triethylenetetramine (TETA)
Marta Ugarte, Marie Brown, Katherine A Hollywood, Garth J Cooper, Paul N Bishop and Warwick B Dunn
Genome Medicine 4 (2012) 35
5 Monitoring the Succinate Dehydrogenase Activity Isolated from Mitochondria by Surface Enhanced Raman Scattering
Katherine A. Hollywood, Iqbal T. Shadi and Royston Goodacre
J. Phys. Chem. C 114 (2010) 7308–7313
4 Phenotypic Characterization of Shewanella oneidensis MR-1 under Aerobic and Anaerobic Growth Conditions by Using Fourier Transform Infrared Spectroscopy and High-Performance Liquid Chromatography Analyses
Hui Wang, Katherine Hollywood, Roger M. Jarvis, Jonathan R. Lloyd, and Royston Goodacre
Applied and Environmental Microbiology 76 (2010) 6266-6276
3 Phenotypic profiling of keloid scars using FT-IR microspectroscopy reveals a unique spectral signature
Katherine A. Hollywood, Marlies Maatje, Iqbal T. Shadi, Alex Henderson, Duncan Angus McGrouther, Royston Goodacre and Ardeshir Bayat
Archives of Dermatological Research 302 (2010) 705-715
2 Predicting human embryo viability: the road to non-invasive analysis of the secretome using metabolic footprinting
Daniel R Brison, Katherine Hollywood, Ruth Arnesen, Royston Goodacre
Reproductive BioMedicine Online 15 (2007) 296–302
1 Metabolomics: Current technologies and future trends
Katherine Hollywood, Daniel R. Brison, Royston Goodacre
PROTEOMICS 6 (2006) 4716–4723