Dr Alex Henderson

Senior Experimental Officer

Honorary Lecturer in Cheminformatics

Honorary Lecturer in Analytical Science

alex.henderson @ manchester.ac.uk

tel: +44 (0)161 306 4462

Follow @AlexHenderson00

PhD on Photoelectron Spectroscopy of Small Metallic Particles, UMIST, 1996
BSc in Chemistry, UMIST, 1988
Certificate in Mathematics, Open University, 2010
Director of SurfaceSpectra Ltd.

Research

My research interests lie in data reduction, working mainly with the results from ToF-SIMS (time of flight secondary ion mass spectrometry) and IR (infra red spectroscopy) experiments in cell chemistry.

Chemometric and cheminformatic approaches are applied to spectra, 2D chemical images and 3D chemical hypercubes to understand the complex chemistries involved in cell structure, metabolism and drug interaction. Work carried out in parallel aims to develop visualisation tools for chemists and biologists working in this area.

Having been in the group for quite a while I find myself involved in most of the projects we have running at any one time. In addition to programming, I find myself helping out around the group, assisting with experimental technique and equipment repair.

In addition to my work in the SIMS field I also work on FTIR data of tissue as a member of Peter Gardner’s research group.

Teaching

I recently taught Instrumentation for Separation Science which was a module of our Analytical and Separation Science Masters programme. My lectures covered the interpretation of mass spectrometric data.

Previously I taught Database Design and Programming on the School of Chemistry’s Cheminformatics Masters programme.

Commercial

I am a director of SurfaceSpectra Ltd. and also write their software.

Other

Recognised UK National Expert

International Standard Organisation (ISO), Technical Committee 201 Surface Chemical Analysis.

TC 201/SC 1 Terminology

TC 201/SC 2 General procedures

TC 201/SC 3 Data management and treatment

TC 201/SC 6 Secondary ion mass spectrometry

Conference organising committees

59th IUVSTA Workshop Surface Chemical Analysis – Improving Data Interpretation by Multivariate and Informatics Techniques
Island of Trinidad, 2010 (Scientific Committee)

The 6th International SPEC conference
Manchester 2010 (Local Organising Committee)

The 15th International Conference on Secondary Ion Mass Spectrometry (SIMS XV)
Manchester 2005 (Local Organising Committee)

Presentations

A selection of recent presentations.

Publications

30	FTIR microscopy of biological cells and tissue: data analysis using resonant Mie scattering (RMieS) EMSC algorithm Paul Bassan, Ashwin Sachdeva, Achim Kohler, Caryn Hughes, Alex Henderson, Jonathan Boyle, Jonathan H. Shanks, Michael Brown, Noel W. Clarke and Peter Gardner Analyst 137 (2012) 1370-1377
29	Three-dimensional mass spectral imaging of HeLa-M cells - sample preparation, data interpretation and visualisation. John S. Fletcher, Sadia Rabbani, Alex Henderson, Nicholas P. Lockyer, John C. Vickerman Rapid Commun. Mass Spectrom. 25 (2011) 925-932
28	Interactive spatio-spectral analysis of three-dimensional mass-spectral (3DxMS) chemical images. Stephen E. Reichenbach, Xue Tian, Robert Lindquist, Qingping Tao, Alex Henderson and John C. Vickerman Surf. Interface Anal. 43 (2011) 529-534
27	Phenotypic profiling of keloid scars using FT-IR microspectroscopy reveals a unique spectral signature Katherine A. Hollywood, Marlies Maatje, Iqbal T. Shadi, Alex Henderson, Duncan Angus McGrouther, Royston Goodacre and Ardeshir Bayat Archives of Dermatological Research 302 (2010) 705-715
26	Classification and Cluster Analysis of Complex Time-of-Flight Secondary Ion Mass Spectrometry for Biological Samples Stephen E. Reichenbach, Xue Tian, Qingping Tao, and Alex Henderson International Conference on Bioinformatics, Computational Biology, Genomics and Chemoinformatics (BCBGC-09) (2009) 78
25	Visualization and analysis of large three-dimensional hyperspectral images Stephen E. Reichenbach, Xue Tian, Robert Lindquist, Qingping Tao, Alex Henderson, and John C. Vickerman Proceedings of SPIE 7341 (2009) 734108
24	Explanatory multivariate analysis of ToF-SIMS spectra for the discrimination of bacterial isolates. Seetharaman Vaidyanathan, John S. Fletcher, Roger M. Jarvis, Alex Henderson, Nicholas P. Lockyer, Royston Goodacre and John C. Vickerman Analyst 134 (2009) 2352-2360
23	A comparison of PCA and MAF for ToF-SIMS image interpretation. Alex Henderson, John S. Fletcher, John C. Vickerman Surf. Interface Anal. 41 (2009) 666-674
22	Factors influencing the discrimination and classification of prostate cancer cell lines by FTIR microspectroscopy. T. J. Harvey, E. Gazi, A. Henderson, R. D. Snook, N. W. Clarke, M. Brown and P. Gardner Analyst 134 (2009) 1083-1091
21	Efficient encoding and rapid decoding for interactive visualization of large three-dimensional hyperspectral chemical images. Reichenbach, Stephen E.; Henderson, Alex; Lindquist, Robert; Tao, Qingping. Rapid Commun. Mass Spectrom. 23 (2009) 1229-1233
20	Classification of fixed urological cells using Raman tweezers. Harvey, Tim J.; Hughes, Caryn; Ward, Andrew D.; Faria, Elsa Correia; Henderson, Alex; Clarke, Noel W.; Brown, Mick D.; Snook, Richard D.; Gardner, Peter. J. Biophotonics 2 (2009) 47-69
19	Spectral discrimination of live prostate and bladder cancer cell lines using Raman optical tweezers Tim J. Harvey, Elsa Correia Faria, Alex Henderson, Ehsan Gazi, Andrew D. Ward, Noel W. Clarke, Michael D. Brown, Richard D. Snook and Peter Gardner Journal of Biomedical Optics 13 (2008) 064004
18	Exploratory analysis of TOF-SIMS data from biological surfaces. Seetharaman Vaidyanathan, John S. Fletcher, Alex Henderson, Nicholas P. Lockyer and John C. Vickerman Applied Surface Science 255 (2008) 1599-1602
17	Uncovering new challenges in bio-analysis with ToF-SIMS. John S. Fletcher, Alex Henderson, Gregory X. Biddulph, Seetharaman Vaidyanathan, Nicholas P. Lockyer and John C. Vickerman Applied Surface Science 255 (2008) 1264-1270
16	A New Dynamic in Mass Spectral Imaging of Single Biological Cells. John S. Fletcher, Sadia Rabbani, Alex Henderson, Paul Blenkinsopp, Steve P. Thompson, Nicholas P. Lockyer, and John C. Vickerman Analytical Chemistry 80 (2008) 9058-9064
15	Discrimination of prostate cancer cells by reflection mode FTIR photoacoustic spectroscopy. Harvey, Tim J.; Henderson, Alex; Gazi, Ehsan; Clarke, Noel W.; Brown, Mick; Faria, Elsa Correia; Snook, Richard D.; Gardner, Peter. Analyst 132 (2007) 292-295
14	The Static SIMS Library version 4 J.C. Vickerman, D. Briggs and A. Henderson SurfaceSpectra, Manchester (2006) CDROM
13	Secondary Ion Mass Spectrometry, SIMS XV. (Proceedings of the Fifteenth International Conference held in UK 12-16 September 2005.) [In: Appl. Surf. Sci., 2006; 252(19)]. Vickerman, John C.; Gilmore, Ian S.; Dowsett, Mark G.; Henderson, Alex; Editors. Applied Surface Science 252 (2006) 922 pp.
12	Rapid discrimination of the causal agents of urinary tract infection using ToF-SIMS with chemometric cluster analysis. Fletcher, John S.; Henderson, Alexander; Jarvis, Roger M.; Lockyer, Nicholas P.; Vickerman, John C.; Goodacre, Royston. Applied Surface Science 252 (2006) 6869-6874
11	ToF-SIMS studies of Bacillus using multivariate analysis with possible identification and taxonomic applications. Thompson, C. E.; Ellis, J.; Fletcher, J. S.; Goodacre, R.; Henderson, A.; Lockyer, N. P.; Vickerman, J. C. Applied Surface Science 252 (2006) 6719-6722
10	Polyethylene terephthalate (PET) bulk film analysis using C60 +, Au3 +, and Au+ primary ion beams. Conlan, X. A.; Gilmore, I. S.; Henderson, A.; Lockyer, N. P.; Vickerman, J. C. Applied Surface Science 252 (2006) 6562-6565
9	ToF-SIMS Studies of Sulfuric Acid Hydrate Films. Fletcher, John S.; Henderson, Alex; Horn, Andrew B.; Vickerman, John C. Journal of Physical Chemistry B 108 (2004) 5960-5966
8	Summary of ISO/TC 201 standard: XXII. ISO 22048: 2004 - surface chemical analysis - information format for static secondary ion mass spectrometry. Gilmore, I. S.; Seah, M. P.; Henderson, A. Surface and Interface Analysis 36 (2004) 1642-1644
7	The role of surface molecular hydrates in the heterogeneous interaction of NH3 with sulfuric acid monohydrate. Nash, Karen L.; Sayer, Robert M.; Couling, Suzanne B.; Fletcher, John; Henderson, Alex; Vickerman, John C.; Horn, Andrew B. Physical Chemistry Chemical Physics 5 (2003) 5101-5107
6	Identification of surface molecular hydrates on solid sulfuric acid films. Couling, Suzanne B.; Nash, Karen L.; Fletcher, John; Henderson, Alex; Vickerman, John C.; Horn, Andrew B. Journal of the American Chemical Society 125 (2003) 13038-13039
5	The Static SIMS Library version 3 J.C. Vickerman, D. Briggs and A. Henderson SurfaceSpectra, Manchester (2002) CDROM
4	NeuroSpectraNet - a self-organising neural network mechanism for interpretation of complex SSIMS spectra. Sanni, O. D.; Henderson, A.; Briggs, D.; Vickerman, J. C. Secondary Ion Mass Spectrometry, SIMS XII, Proceedings of the International Conference on Secondary Ion Mass Spectrometry, 12th, Brussels, Belgium, Sept. 5-10, 1999 (2000) 805-808
3	The Static SIMS Library version 2 J.C. Vickerman, D. Briggs and A. Henderson SurfaceSpectra, Manchester (1999) pp1200 & CDROM
2	The Static SIMS Library J.C. Vickerman, D. Briggs and A. Henderson SurfaceSpectra, Manchester (1997) pp1000 & CDROM
1	The effect of corona treatments on the hygral expansion of wool worsted fabrics. Carr, C. M.; Dodd, K. J.; Connors, M. A.; Henderson, A.; Vickerman, J. Journal of the Society of Dyers and Colourists 110 (1994) 383-6

Acknowledged in…

Here are links to articles I have been acknowledged in.

	ToF-SIMS PC-DFA analysis of prostate cancer cell lines. M.J. Baker, E. Gazi, M.D. Brown, N.W. Clarke, J.C. Vickerman and N.P. Lockyer Appl. Surf. Sci. 255 (2008) 1084-1087
	*TOF-SIMS investigation of Streptomyces coelicolor, a mycelial bacterium.* Seetharaman Vaidyanathan, John S. Fletcher, Nicholas P. Lockyer and John C. Vickerman Appl. Surf. Sci. 255 (2008) 922-925
	*Subsurface Biomolecular Imaging of Streptomyces coelicolor* Using Secondary Ion Mass Spectrometry.** Seetharaman Vaidyanathan, John S. Fletcher, Roy Goodacre, Nicholas P. Lockyer, Jason Micklefield, and John C. Vickerman Analytical Chemistry 80 (2008) 1942-1951
	Depth Profiling Brain Tissue Sections with a 40 keV C60+ Primary Ion Beam. Emrys A. Jones, Nicholas P. Lockyer, John C. Vickerman Analytical Chemistry 80 (2008) 2125-2132
	*TOF-SIMS 3D Biomolecular Imaging of Xenopus laevis* Oocytes Using Buckminsterfullerene (C60) Primary Ions.** Fletcher, John S.; Lockyer, Nicholas P.; Vaidyanathan, Seetharaman; Vickerman, John C. Analytical Chemistry 79 (2007) 2199-2206

Named on…

Here are links to grants on which I have been funded.

2011	MaDAM into Sustainable Service (MiSS) Lorraine Beard, PI (Head of Information Services, JRUL), Project Manager June Finch JISC £245k + £730k institutional support October 2011 – March 2013 (extension of the MaDAM project)
2011	Infrared Imaging for Diagnosis and Prediction of the Biopotental of Low and Intermediate Risk Prostate Cancer Gardner (PI), Brown, Clarke EPSRC £476k April 2011 – March 2014
2011	MIB CDFA – Application of novel imaging technologies towards quantitative analytical phenotyping of single cells Flitsch (PI), Goddard, Vickerman, Bayat, Westerhoff, Yuan, Webb, Gardner, Fielden, Goodacre, Lockyer, Fletcher, Blanch EPSRC £201k January 2011 – January 2012
2009	A new dynamic in mass spectral imaging for biological systems Vickerman (PI), Lockyer, Fletcher EPSRC £740k June 2009 – May 2012
2009	A combined micro fluidic single cell SRIR microscopy stage for use at Diamond Gardner (PI), Cinque, Fielden, Goddard EPSRC £170k January 2009 – December 2012
2007	Enhancing new developments in ToF-SIMS through researcher exchanges Vickerman, Lockyer, Webb (Surrey) EPSRC Collaborating for Success Through People Award £110k [more information...]
2005	New Developments in ToF-SIMS Surface Mass Spectrometry with ATR-IR Spectroscopy Vickerman (PI), Lockyer, Gardner, Horn, Goodacre; Webb and Kirkby (Surrey). EPSRC Critical Mass Award £2M October 2005 – Sept 2009
2004	A High Resolution ToF-SIMS Microscope for Biology Vickerman (PI), Lockyer, Goodacre and Kell. BBSRC £428k August 2004 – December 2007
2000	Development of ToF-SIMS instrumentation and methodology for chemical microscopy of cellular systems Vickerman, Lockyer and Stephens EPSRC Life Sciences Interface £312k June 2000 – June 2003
1996	Wiley Static SIMS Library – Ongoing funding for updates £13,000/year
1994	Extension to Static SIMS Library Grant July 1994 to May 1995 – John Wiley & Sons, £17,000
1992	Support from John Wiley & Sons for the launch costs (>£50k) of an International Static SIMS Database.

Surface Analysis Research Centre

University of Manchester, UK